不同降水下覆盖与缓释肥减量对油菜土壤微生物群落结构的影响

为探讨沟垄集雨、秸秆覆盖和减量缓释肥集成技术对油菜土壤微生物群落结构的影响,在大田定位试验条件下,设置3种栽培方式（秸秆覆盖、沟垄集雨、传统平作）和4种施肥水平（习惯施肥、缓释肥减量20%、缓释肥减量40%、不施肥）,比较分析2016~2017年（多雨年份）和2017~2018年（季节性干旱年份）这2种不同降水年型下油菜土壤微生物群落结构对集成技术的响应.结果表明,不同降雨条件下,秸秆覆盖+缓释肥减量20%（J80）与沟垄覆盖+缓释肥减量20%（M80）较习惯栽培（PC）更利于土壤微生物生长.在多雨年型和季节性干旱年型下,J80与M80能平均分别提高微生物量碳9.94%和10.32%;提高土壤微生物量氮2.38%和1.19%.多雨年型下,土壤微生物磷酸脂肪酸（PLFA）总量较干旱年型降低30.75%,覆盖能有效提高土壤PLFA质量摩尔浓度.多雨年型下土壤细菌、真菌PLFA质量摩尔浓度较干旱年型平均减少33.67%和53.21%;而放线菌PLFA增加13.04%.微生物群落对异常降水具有敏感响应.多雨气候会造成细菌/真菌升高;干旱气候则会增加直链饱和脂肪酸/直链单不饱和脂肪酸及直链单不饱和脂肪酸/环丙烷脂肪酸.通过秸秆覆盖与缓释肥的集成技术,能稳定异常降水下的土壤微环境,缓解水分和养分胁迫,为油菜稳产和可持续发展提供有效途径.     

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Ultrasensitive and selective 4-aminophenol chemical sensor development based on nickel oxide nanoparticles decorated carbon nanotube nanocomposites for green environment

Nickel oxide nanoparticles decorated carbon nanotube nanocomposites(Ni O·CNT NCs)were prepared in a basic medium by using facile wet-chemical routes. The optical,morphological, and structural properties of Ni O·CNT NCs were characterized using Fourier transformed infra-red(FT-IR), Ultra-violet visible(UV/Vis) spectroscopy, field-emission scanning electron microscopy(FESEM), X-ray energy dispersed spectroscopy(XEDS), X-ray photoelectron spectroscopy(XPS), and powder X-ray diffraction(XRD) methods. Selective4-aminophenol(4-AP) chemical sensor was developed by a flat glassy carbon electrode(GCE, surface area: 0.0316 cm~2) fabricated with a thin-layer of NCs. Electrochemical responses including higher sensitivity, large dynamic range(LDR), limit of detection(LOD), and long-term stability towards 4-AP were obtained using the fabricated chemical sensors. The calibration curve was found linear(R~2= 0.914) over a wide range of 4-AP concentration(0.1 nmol/L–0.1 mol/L). In perspective of slope(2 × 10~(-5)μA/μM), LOD and sensitivity were calculated as 15.0 ± 0.1 pM and ~ 6.33 × 10~(-4)μA/(μM·cm) respectively. The synthesized Ni O·CNT NCs using a wet-chemical method is a significant route for the development of ultrasensitive and selective phenolic sensor based on nano-materials for environmental toxic substances. It is suggested that a pioneer and selective development of 4-AP sensitive sensor using Ni O·CNT NCs by a facile and reliable current vs voltage(I–V)method for the major application of toxic agents in biological, green environmental, and health-care fields in near future.